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LIF Protein (AA 24-203) (His tag)

LIF Origine: Souris Hôte: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
N° du produit ABIN2870754
  • Antigène Voir toutes LIF Protéines
    LIF (Leukemia Inhibitory Factor (LIF))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 24-203
    Origine
    • 22
    • 14
    • 4
    • 3
    • 2
    • 2
    • 1
    Souris
    Source
    • 21
    • 15
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Purification/Conjugué
    Cette LIF protéine est marqué à la His tag.
    Séquence
    AA 24-203
    Attributs du produit
    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.7 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    Pureté
    >90 % as determined by SDS-PAGE.
    niveau d'endotoxine
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product LIF Protéine
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Conseil sur la manipulation
    Please avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
  • Antigène
    LIF (Leukemia Inhibitory Factor (LIF))
    Autre désignation
    LIF (LIF Produits)
    Synonymes
    CDF Protein, DIA Protein, HILDA Protein, MLPLI Protein, LIF, interleukin 6 family cytokine Protein, leukemia inhibitory factor Protein, LIF Protein, Lif Protein
    Sujet
    Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.
    Poids moléculaire
    20.8 kDa
    NCBI Accession
    NP_032527
    UniProt
    P09056
    Pathways
    Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
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