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Ubiquitin-Conjugating Enzyme E2I (UBE2I) (AA 2-158) protein (His tag) Protein

Crystallography grade UBE2I Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3075177
$7,651.94
Plus frais de port $45.00 et $20.00 pour carboglace
Destination: Etats-Unis
Envoi sous 50 jours ouvrables
  • Antigène Voir toutes UBE2I Protéines
    UBE2I
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-158
    Origine
    • 12
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Source
    • 7
    • 7
    • 4
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette UBE2I protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    SGIALSRLAQ ERKAWRKDHP FGFVAVPTKN PDGTMNLMNW ECAIPGKKGT PWEGGLFKLR MLFKDDYPSS PPKCKFEPPL FHPNVYPSGT VCLSILEEDK DWRPAITIKQ ILLGIQELLN EPNIQDPAQA EAYTIYCQNR VEYEKRVRAQ AKKFAPS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human UBE2I Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    UBE2I
    Autre désignation
    UBE2I (UBE2I Produits)
    Synonymes
    C358B7.1, P18, UBC9, ubc9, ube2ia, zUbc9, 5830467E05Rik, F830028O17Rik, Ubce2i, Ubce9, UbcE2A, ubce9, ubiquitin conjugating enzyme E2 I, ubiquitin-conjugating enzyme E2Ib, ubiquitin-conjugating enzyme E2L, ubiquitin-conjugating enzyme E2I, ubiquitin conjugating enzyme E2I S homeolog, SUMO-conjugating enzyme UBC9, UBE2I, ube2ib, UBE2L, Ube2i, ube2i.S, LOC108703134
    Sujet
    Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. {ECO:0000269|PubMed:11451954, ECO:0000269|PubMed:15809060, ECO:0000269|PubMed:17466333, ECO:0000269|PubMed:19638400, ECO:0000269|PubMed:19744555, ECO:0000269|PubMed:20077568, ECO:0000269|PubMed:26524494, ECO:0000269|PubMed:8668529}.
    Poids moléculaire
    18.8 kDa Including tag.
    UniProt
    P63279
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
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