PRNP Protein (AA 23-230) (His tag)
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- Antigène Voir toutes PRNP Protéines
- PRNP (Prion Protein (PRNP))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 23-230
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette PRNP protéine est marqué à la His tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- Séquence
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KKRPKPGGWN TGGSRYPGQG SPGGNRYPPQ GGGGWGQPHG GGWGQPHGGG WGQPHGGGWG QPHGGGWGQG GGTHSQWNKP SKPKTNMKHM AGAAAAGAVV GGLGGYMLGS AMSRPIIHFG SDYEDRYYRE NMHRYPNQVY YRPMDEYSNQ NNFVHDCVNI TIKQHTVTTT TKGENFTETD VKMMERVVEQ MCITQYERES QAYYQRGS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PRNP Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product PRNP Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- PRNP (Prion Protein (PRNP))
- Autre désignation
- PRNP (PRNP Produits)
- Synonymes
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ASCR Protein, AltPrP Protein, CD230 Protein, CJD Protein, GSS Protein, KURU Protein, PRIP Protein, PrP Protein, PrP27-30 Protein, PrP33-35C Protein, PrPc Protein, p27-30 Protein, Prn Protein, Prn-p Protein, prp Protein, cjd Protein, prion Protein, prp(c) Protein, MGC84114 Protein, PRP Protein, PRPC Protein, Prp Protein, SIP Protein, PRNP Protein, AA960666 Protein, AI325101 Protein, PrP
Protein, PrPC Protein, PrPSc Protein, Prn-i Protein, Sinc Protein, prP27-30 Protein, prP33-35C Protein, PrP-1 Protein, PrP1 Protein, prnprs1 Protein, stPrP-1 Protein, zgc:123004 Protein, prion protein Protein, prion protein S homeolog Protein, prion protein b Protein, PRNP Protein, Prnp Protein, prnp.S Protein, prnpb Protein - Sujet
- Its primary physiological function is unclear. Has cytoprotective activity against internal or environmental stresses. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (PubMed:12732622, PubMed:19936054, PubMed:20564047). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity). {ECO:0000250|UniProtKB:P04925, ECO:0000269|PubMed:12732622, ECO:0000269|PubMed:19936054, ECO:0000269|PubMed:20564047, ECO:0000305}.
- Poids moléculaire
- 23.7 kDa Including tag.
- UniProt
- P04156
- Pathways
- Transition Metal Ion Homeostasis, Activated T Cell Proliferation
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