PSMD8 Protein (AA 1-350) (His tag)

N° du produit ABIN3086673

Détail du produit

Antigène: PSMD8 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8))

Type de proteíne: Recombinant

Attributs du protein: AA 1-350

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette PSMD8 protéine est marqué à la His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MFIKGRAPRA PPRERRRATR GGLRQVVAPP RALGSTSRPH FRRASVCRRR CRKSGGLLAA SRKMAAAAVN GAAGFSSSGP AATSGAVLQA ATGMYEQLKG EWNRKSPNLS KCGEELGRLK LVLLELNFLP TTGTKLTKQQ LILARDILEI GAQWSILRKD IPSFERYMAQ LKCYYFDYKE QLPESAYMHQ LLGLNLLFLL SQNRVAEFHT ELERLPAKDI QTNVYIKHPV SLEQYLMEGS YNKVFLAKGN IPAESYTFFI DILLDTIRDE IAGCIEKAYE KILFTEATRI LFFNTPKKMT DYAKKRGWVL GPNNYYSFAS QQQKPEDTTI PSTELAKQVI EYARQLEMIV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PSMD8 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PSMD8 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8))

Autre désignation: PSMD8 (PSMD8 Produits)

Synonymes: HIP6 Protein, HYPF Protein, Nin1p Protein, Rpn12 Protein, S14 Protein, p31 Protein, hip6 Protein, hypf Protein, nin1p Protein, rpn12 Protein, psmd8b Protein, 6720456J22Rik Protein, AA407360 Protein, AL033291 Protein, AL033322 Protein, AL033323 Protein, C76433 Protein, psmd8 Protein, psmd8a Protein, fa93g07 Protein, fb17c09 Protein, fb49a10 Protein, zgc:86762 Protein, wu:fa93g07 Protein, wu:fb17c09 Protein, wu:fb49a10 Protein, PSMD8 Protein, proteasome 26S subunit, non-ATPase 8 Protein, proteasome 26S subunit, non-ATPase 8 S homeolog Protein, proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 Protein, proteasome 26S subunit, non-ATPase 8 L homeolog Protein, proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 pseudogene Protein, PSMD8 Protein, psmd8.S Protein, Psmd8 Protein, psmd8.L Protein, psmd8 Protein, LOC468490 Protein

Sujet: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Necessary for activation of the CDC28 kinase.

Poids moléculaire: 40.6 kDa Including tag.

UniProt: P48556

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Proton Transport, Synthesis of DNA, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway