ARHGEF12 Protein (AA 2-1544) (His tag)

N° du produit ABIN3089238

Détail du produit

Antigène: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1544

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette ARHGEF12 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: SGTQSTITDR FPLKKPIRHG SILNRESPTD KKQKVERIAS HDFDPTDSSS KKTKSSSEES RSEIYGLVQR CVIIQKDDNG FGLTVSGDNP VFVQSVKEDG AAMRAGVQTG DRIIKVNGTL VTHSNHLEVV KLIKSGSYVA LTVQGRPPGS PQIPLADSEV EPSVIGHMSP IMTSPHSPGA SGNMERITSP VLMGEENNVV HNQKVEILRK MLQKEQERLQ LLQEDYNRTP AQRLLKEIQE AKKHIPQLQE QLSKATGSAQ DGAVVTPSRP LGDTLTVSEA ETDPGDVLGR TDCSSGDASR PSSDNADSPK SGPKERIYLE ENPEKSETIQ DTDTQSLVGS PSTRIAPHII GAEDDDFGTE HEQINGQCSC FQSIELLKSR PAHLAVFLHH VVSQFDPATL LCYLYSDLYK HTNSKETRRI FLEFHQFFLD RSAHLKVSVP DEMSADLEKR RPELIPEDLH RHYIQTMQER VHPEVQRHLE DFRQKRSMGL TLAESELTKL DAERDKDRLT LEKERTCAEQ IVAKIEEVLM TAQAVEEDKS STMQYVILMY MKHLGVKVKE PRNLEHKRGR IGFLPKIKQS MKKDKEGEEK GKRRGFPSIL GPPRRPSRHD NSAIGRAMEL QKARHPKHLS TPSSVSPEPQ DSAKLRQSGL ANEGTDAGYL PANSMSSVAS GASFSQEGGK ENDTGSKQVG ETSAPGDTLD GTPRTLNTVF DFPPPPLDQV QEEECEVERV TEHGTPKPFR KFDSVAFGES QSEDEQFEND LETDPPNWQQ LVSREVLLGL KPCEIKRQEV INELFYTERA HVRTLKVLDQ VFYQRVSREG ILSPSELRKI FSNLEDILQL HIGLNEQMKA VRKRNETSVI DQIGEDLLTW FSGPGEEKLK HAAATFCSNQ PFALEMIKSR QKKDSRFQTF VQDAESNPLC RRLQLKDIIP TQMQRLTKYP LLLDNIAKYT EWPTEREKVK KAADHCRQIL NYVNQAVKEA ENKQRLEDYQ RRLDTSSLKL SEYPNVEELR NLDLTKRKMI HEGPLVWKVN RDKTIDLYTL LLEDILVLLQ KQDDRLVLRC HSKILASTAD SKHTFSPVIK LSTVLVRQVA TDNKALFVIS MSDNGAQIYE LVAQTVSEKT VWQDLICRMA ASVKEQSTKP IPLPQSTPGE GDNDEEDPSK LKEEQHGISV TGLQSPDRDL GLESTLISSK PQSHSLSTSG KSEVRDLFVA ERQFAKEQHT DGTLKEVGED YQIAIPDSHL PVSEERWALD ALRNLGLLKQ LLVQQLGLTE KSVQEDWQHF PRYRTASQGP QTDSVIQNSE NIKAYHSGEG HMPFRTGTGD IATCYSPRTS TESFAPRDSV GLAPQDSQAS NILVMDHMIM TPEMPTMEPE GGLDDSGEHF FDAREAHSDE NPSEGDGAVN KEEKDVNLRI SGNYLILDGY DPVQESSTDE EVASSLTLQP MTGIPAVEST HQQQHSPQNT HSDGAISPFT PEFLVQQRWG AMEYSCFEIQ SPSSCADSQS QIMEYIHKIE ADLEHLKKVE ESYTILCQRL AGSALTDKHS DKS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ARHGEF12 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Autre désignation: ARHGEF12 (ARHGEF12 Produits)

Synonymes: larg Protein, ARHGEF12 Protein, Larg Protein, pro2792 Protein, LARG Protein, PRO2792 Protein, 2310014B11Rik Protein, AU019857 Protein, mKIAA0382 Protein, Rho guanine nucleotide exchange factor 12 S homeolog Protein, Rho guanine nucleotide exchange factor 12 Protein, Rho guanine nucleotide exchange factor (GEF) 12 Protein, arhgef12.S Protein, ARHGEF12 Protein, arhgef12 Protein, Arhgef12 Protein

Sujet: May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. {ECO:0000269|PubMed:11094164}.

Poids moléculaire: 174.1 kDa Including tag.

UniProt: Q9NZN5

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling