DLGAP5 Protein (AA 1-846) (Strep Tag)
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- Antigène Voir toutes DLGAP5 Protéines
- DLGAP5 (Discs, Large (Drosophila) Homolog-Associated Protein 5 (DLGAP5))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-846
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Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette DLGAP5 protéine est marqué à la Strep Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Séquence
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MSSSHFASRH RKDISTEMIR TKIAHRKSLS QKENRHKEYE RNRHFGLKDV NIPTLEGRIL VELDETSQGL VPEKTNVKPR AMKTILGDQR KQMLQKYKEE KQLQKLKEQR EKAKRGIFKV GRYRPDMPCF LLSNQNAVKA EPKKAIPSSV RITRSKAKDQ MEQTKIDNES DVRAIRPGPR QTSEKKVSDK EKKVVQPVMP TSLRMTRSAT QAAKQVPRTV SSTTARKPVT RAANENEPEG KVPSKGRPAK NVETKPDKGI SCKVDSEENT LNSQTNATSG MNPDGVLSKM ENLPEINTAK IKGKNSFAPK DFMFQPLDGL KTYQVTPMTP RSANAFLTPS YTWTPLKTEV DESQATKEIL AQKCKTYSTK TIQQDSNKLP CPLGPLTVWH EEHVLNKNEA TTKNLNGLPI KEVPSLERNE GRIAQPHHGV PYFRNILQSE TEKLTSHCFE WDRKLELDIP DDAKDLIRTA VGQTRLLMKE RFKQFEGLVD DCEYKRGIKE TTCTDLDGFW DMVSFQIEDV IHKFNNLIKL EESGWQVNNN MNHNMNKNVF RKKVVSGIAS KPKQDDAGRI AARNRLAAIK NAMRERIRQE ECAETAVSVI PKEVDKIVFD AGFFRVESPV KLFSGLSVSS EGPSQRLGTP KSVNKAVSQS RNEMGIPQQT TSPENAGPQN TKSEHVKKTL FLSIPESRSS IEDAQCPGLP DLIEENHVVN KTDLKVDCLS SERMSLPLLA GGVADDINTN KKEGISDVVE GMELNSSITS QDVLMSSPEK NTASQNSILE EGETKISQSE LFDNKSLTTE CHLLDSPGLN CSNPFTQLER RHQEHARHIS FGGNLITFSP LQPGEF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product DLGAP5 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- DLGAP5 (Discs, Large (Drosophila) Homolog-Associated Protein 5 (DLGAP5))
- Autre désignation
- DLGAP5 (DLGAP5 Produits)
- Synonymes
- DLG7 Protein, dlg7 Protein, sb:cb647 Protein, zgc:92146 Protein, wu:fc96g08 Protein, wu:fe11c02 Protein, HURP Protein, C77459 Protein, C86398 Protein, Dap-5 Protein, Dlg7 Protein, Hurp Protein, mKIAA0008 Protein, DLG associated protein 5 Protein, discs, large (Drosophila) homolog-associated protein 5 Protein, DLGAP5 Protein, dlgap5 Protein, Dlgap5 Protein
- Sujet
- Disks large-associated protein 5 (DAP-5) (Discs large homolog 7) (Disks large-associated protein DLG7) (Hepatoma up-regulated protein) (HURP),FUNCTION: Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells. {ECO:0000269|PubMed:12527899, ECO:0000269|PubMed:14699157, ECO:0000269|PubMed:15145941}.
- Poids moléculaire
- 95.1 kDa
- UniProt
- Q15398
- Pathways
- M Phase
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