PCSK5 Protein (AA 115-1743) (His tag)

N° du produit ABIN3094407

Détail du produit

Antigène: PCSK5 (Proprotein Convertase Subtilisin/kexin Type 5 (PCSK5))

Type de proteíne: Recombinant

Attributs du protein: AA 115-1743

Origine: Humain

Source: Cellules d'insectes

Purification/Conjugué: Cette PCSK5 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: DYDFSRAQST YFNDPKWPSM WYMHCSDNTH PCQSDMNIEG AWKRGYTGKN IVVTILDDGI ERTHPDLMQN YDALASCDVN GNDLDPMPRY DASNENKHGT RCAGEVAAAA NNSHCTVGIA FNAKIGGVRM LDGDVTDMVE AKSVSFNPQH VHIYSASWGP DDDGKTVDGP APLTRQAFEN GVRMGRRGLG SVFVWASGNG GRSKDHCSCD GYTNSIYTIS ISSTAESGKK PWYLEECSST LATTYSSGES YDKKIITTDL RQRCTDNHTG TSASAPMAAG IIALALEANP FLTWRDVQHV IVRTSRAGHL NANDWKTNAA GFKVSHLYGF GLMDAEAMVM EAEKWTTVPR QHVCVESTDR QIKTIRPNSA VRSIYKASGC SDNPNRHVNY LEHVVVRITI THPRRGDLAI YLTSPSGTRS QLLANRLFDH SMEGFKNWEF MTIHCWGERA AGDWVLEVYD TPSQLRNFKT PGKLKEWSLV LYGTSVQPYS PTNEFPKVER FRYSRVEDPT DDYGTEDYAG PCDPECSEVG CDGPGPDHCN DCLHYYYKLK NNTRICVSSC PPGHYHADKK RCRKCAPNCE SCFGSHGDQC MSCKYGYFLN EETNSCVTHC PDGSYQDTKK NLCRKCSENC KTCTEFHNCT ECRDGLSLQG SRCSVSCEDG RYFNGQDCQP CHRFCATCAG AGADGCINCT EGYFMEDGRC VQSCSISYYF DHSSENGYKS CKKCDISCLT CNGPGFKNCT SCPSGYLLDL GMCQMGAICK DGEYVDEHGH CQTCEASCAK CQGPTQEDCT TCPMTRIFDD GRCVSNCPSW KFEFENQCHP CHHTCQRCQG SGPTHCTSCG ADNYGREHFL YQGECGDSCP EGHYATEGNT CLPCPDNCEL CHSVHVCTRC MKGYFIAPTN HTCQKLECGQ GEVQDPDYEE CVPCEEGCLG CSLDDPGTCT SCAMGYYRFD HHCYKTCPEK TYSEEVECKA CDSNCGSCDQ NGCYWCEEGF FLLGGSCVRK CGPGFYGDQE MGECESCHRA CETCTGPGHD ECSSCQEGLQ LLRGMCVHAT KTQEEGKFWN DILRKLQPCH SSCKTCNGSA TLCTSCPKGA YLLAQACVSS CPQGTWPSVR SGSCENCTEA CAICSGADLC KKCQMQPGHP LFLHEGRCYS KCPEGSYAED GICERCSSPC RTCEGNATNC HSCEGGHVLH HGVCQENCPE RHVAVKGVCK HCPEMCQDCI HEKTCKECTP EFFLHDDMCH QSCPRGFYAD SRHCVPCHKD CLECSGPKAD DCELCLESSW VLYDGLCLEE CPAGTYYEKE TKECRDCHKS CLTCSSSGTC TTCQKGLIMN PRGSCMANEK CSPSEYWDED APGCKPCHVK CFHCMGPAED QCQTCPMNSL LLNTTCVKDC PEGYYADEDS NRCAHCHSSC RTCEGRHSRQ CHSCRPGWFQ LGKECLLQCR EGYYADNSTG RCERCNRSCK GCQGPRPTDC LSCDRFFFLL RSKGECHRSC PDHYYVEQST QTCERCHPTC DQCKGKGALN CLSCVWSYHL MGGICTSDCL VGEYRVGEGE KFNCEKCHES CMECKGPGAK NCTLCPANLV LHMDDSHCLH CCNTSDPPSA QECCDCQDTT DECILRTSKV RPATEHFKT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PCSK5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PCSK5 (Proprotein Convertase Subtilisin/kexin Type 5 (PCSK5))

Autre désignation: PCSK5 (PCSK5 Produits)

Synonymes: PC5 Protein, PC6 Protein, PC6A Protein, SPC6 Protein, subtilase Protein, PC5/6A Protein, PC5A Protein, b2b1549Clo Protein, b2b585Clo Protein, Pc5 Protein, fur2 Protein, spc6 Protein, spc6A Protein, MGC98915 Protein, PCSK5 Protein, pc6 Protein, spc6a Protein, pcsk5 Protein, zgc:165659 Protein, proprotein convertase subtilisin/kexin type 5 Protein, proprotein convertase subtilisin/kexin type 5 S homeolog Protein, proprotein convertase subtilisin/kexin type 5b Protein, PCSK5 Protein, Pcsk5 Protein, pcsk5.S Protein, LOC528098 Protein, pcsk5 Protein, pcsk5b Protein

Sujet: Likely to represent a widespread endoprotease activity within the constitutive and regulated secretory pathway. Capable of cleavage at the RX(K/R)R consensus motif. Plays an essential role in pregnancy establishment by proteolytic activation of a number of important factors such as BMP2, CALD1 and alpha-integrins. {ECO:0000269|PubMed:19764806, ECO:0000269|PubMed:20555025, ECO:0000269|PubMed:22740495}.

Poids moléculaire: 181.4 kDa Including tag.

UniProt: Q92824

Pathways: Neurotrophin Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones