TBC1D2 Protein (AA 1-928) (Strep Tag)
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- Antigène Voir toutes TBC1D2 Protéines
- TBC1D2 (TBC1 Domain Family, Member 2 (TBC1D2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-928
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Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette TBC1D2 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MEGAGENAPE SSSSAPGSEE SARDPQVPPP EEESGDCARS LEAVPKKLCG YLSKFGGKGP IRGWKSRWFF YDERKCQLYY SRTAQDANPL DSIDLSSAVF DCKADAEEGI FEIKTPSRVI TLKAATKQAM LYWLQQLQMK RWEFHNSPPA PPATPDAALA GNGPVLHLEL GQEEAELEEF LCPVKTPPGL VGVAAALQPF PALQNISLKH LGTEIQNTMH NIRGNKQAQG TGHEPPGEDS PQSGEPQREE QPLASDASTP GREPEDSPKP APKPSLTISF AQKAKRQNNT FPFFSEGITR NRTAQEKVAA LEQQVLMLTK ELKSQKELVK ILHKALEAAQ QEKRASSAYL AAAEDKDRLE LVRHKVRQIA ELGRRVEALE QERESLAHTA SLREQQVQEL QQHVQLLMDK NHAKQQVICK LSEKVTQDFT HPPDQSPLRP DAANRDFLSQ QGKIEHLKDD MEAYRTQNCF LNSEIHQVTK IWRKVAEKEK ALLTKCAYLQ ARNCQVESKY LAGLRRLQEA LGDEASECSE LLRQLVQEAL QWEAGEASSD SIELSPISKY DEYGFLTVPD YEVEDLKLLA KIQALESRSH HLLGLEAVDR PLRERWAALG DLVPSAELKQ LLRAGVPREH RPRVWRWLVH LRVQHLHTPG CYQELLSRGQ AREHPAARQI ELDLNRTFPN NKHFTCPTSS FPDKLRRVLL AFSWQNPTIG YCQGLNRLAA IALLVLEEEE SAFWCLVAIV ETIMPADYYC NTLTASQVDQ RVLQDLLSEK LPRLMAHLGQ HHVDLSLVTF NWFLVVFADS LISNILLRVW DAFLYEGTKV VFRYALAIFK YNEKEILRLQ NGLEIYQYLR FFTKTISNSR KLMNIAFNDM NPFRMKQLRQ LRMVHRERLE AELRELEQLK AEYLERRASR RRAVSEGCAS EDEVEGEA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product TBC1D2 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- TBC1D2 (TBC1 Domain Family, Member 2 (TBC1D2))
- Autre désignation
- TBC1D2 (TBC1D2 Produits)
- Synonymes
- PARIS-1 Protein, PARIS1 Protein, TBC1D2A Protein, A630005A06Rik Protein, BC022630 Protein, Gm1037 Protein, TBC1 domain family member 2 Protein, TBC1 domain family, member 2 Protein, TBC1D2 Protein, Tbc1d2 Protein
- Sujet
- TBC1 domain family member 2A (Armus) (Prostate antigen recognized and identified by SEREX 1) (PARIS-1),FUNCTION: Acts as a GTPase-activating protein for RAB7A. Signal effector acting as a linker between RAC1 and RAB7A, leading to RAB7A inactivation and subsequent inhibition of cadherin degradation and reduced cell-cell adhesion. {ECO:0000269|PubMed:20116244}.
- Poids moléculaire
- 105.4 kDa
- UniProt
- Q9BYX2
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