NR4A1 Protein (AA 1-601) (Strep Tag)

N° du produit ABIN3132921

Détail du produit

Antigène: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-601

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette NR4A1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MPCIQAQYGT PATSPGPRDH LTGDPLALEF GKPTMDLASP ETAPAAPATL PSFSTFMDGY TGEFDTFLYQ LPGTTQPCSS ACSSASSTSS SSSSATSPAS ASFKFEDFQV YGCYPGTLSG PLDETLSSSG SEYYGSPCSA PSPSTPNFQP SQLSPWDGSF GHFSPSQTYE GLWAWTEQLP KASSGPPPPP TFFSFSPPTG PSPSLAQSSL KLFPPPATHQ LGEGESYSMP AAFPGLAPTS PNRDTSGILD APVTSTKSRS GASGGSEGRC AVCGDNASCQ HYGVRTCEGC KGFFKRTVQK SAKYICLANK DCPVDKRRRN RCQFCRFQKC LAVGMVKEVV RTDSLKGRRG RLPSKPKQPP DASPTNLLTS LIRAHLDSGP STAKLDYSKF QELVLPRFGK EDAGDVQQFY DLLSGSLDVI RKWAEKIPGF IELCPGDQDL LLESAFLELF ILRLAYRSKP GEGKLIFCSG LVLHQLQCAR GFGDWIDNIL AFSRSLHSLG VDVPAFACLS ALVLITDRHG LQDPRRVEEL QNRIASCLKE HMATVAGDPQ PASCLSRLLG KLPELRTLCT QGLQRIFCLK LEDLVPPPPI VDKIFMDTLS F
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))

Autre désignation: Nr4a1 (NR4A1 Produits)

Synonymes: GFRP1 Protein, HMR Protein, N10 Protein, NAK-1 Protein, NGFIB Protein, NP10 Protein, NUR77 Protein, TR3 Protein, NGFI-B Protein, Gfrp Protein, Hbr-1 Protein, Hbr1 Protein, Hmr Protein, TIS1 Protein, nur77 Protein, Ngfi-b Protein, Nur77 Protein, nuclear receptor subfamily 4 group A member 1 Protein, nuclear receptor subfamily 4, group A, member 1 Protein, NR4A1 Protein, Nr4a1 Protein

Sujet: Nuclear receptor subfamily 4 group A member 1 (Nuclear hormone receptor NUR/77) (Nuclear protein N10) (Orphan nuclear receptor HMR),FUNCTION: Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3' (PubMed:37001519). Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain (By similarity). Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (By similarity). Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA) (PubMed:30134173). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter (PubMed:30134173). May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration (By similarity). Plays a role in the vascular response to injury (PubMed:22427340). {ECO:0000250|UniProtKB:P22736, ECO:0000250|UniProtKB:P22829, ECO:0000269|PubMed:22427340, ECO:0000269|PubMed:30134173, ECO:0000269|PubMed:37001519}., FUNCTION: In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7. {ECO:0000269|PubMed:37001519}.

Poids moléculaire: 64.7 kDa

UniProt: P12813

Pathways: Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway