MCM6 Protein (AA 1-821) (Strep Tag)

N° du produit ABIN3134682

Détail du produit

Antigène: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Type de proteíne: Recombinant

Attributs du protein: AA 1-821

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MCM6 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MDLAAAAEPG AGSQHPEVRD EVAEKCQKLF LDFLEEFQGS DGEIKYLQFA EELIRPERNT LVVSFADLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD RKEIPFAKDF YVAFQDLPTR HKIRELTSSR IGLLTRISGQ VVRTHPVHPE LVSGTFLCLD CQTVIKDVEQ QFKYTQPNIC RNPVCANRKR FLLDTNKSRF VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD RCDFTGALIV VPDVSKLSTP GARAETNSRV SGADGYETEG IRGLRALGVR DLSYRLVFLA CHVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL CTSLFPTIHG NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST AKSQFLKHVD EFSPRAVYTS GKASSAAGLT AAVVRDEESH EFVIEAGALM LADNGVCCID EFDKMDMRDQ VAIHEAMEQQ TISITKAGVK ATLNARTSIL AAANPVSGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC NEVTDYAIAR RIVDLHSRIE ESIDRVYSLD DIRRYLLFAR QFKPKISKES EDFIVEQYKR LRQRDGSGVT KSSWRITVRQ LESMIRLSES MARMHCCDEV QPKHVKEAFR LLNKSIIRVE TPDVNLDQEE EIQMETDEGQ GGVNGHADSP APVNRFNGSS EDASQETVSK PSLRLGFAEY CRISNLIVLH LRKMEEEEDE SALKRSELVN WYLKEIESEI DSEEELINKK TIIEKVVHRL THYDHVLIEL TQAGLKGSSE GSESYEEDPY LVVNPNYLLE D
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MCM6 (Minichromosome Maintenance Complex Component 6 (MCM6))

Autre désignation: Mcm6 (MCM6 Produits)

Synonymes: CG4039 Protein, DmMCM6 Protein, DmeMCM6 Protein, Dmel\\CG4039 Protein, MCM6 Protein, McM6 Protein, fs(1)K1214 Protein, mcm6 Protein, MCG40308 Protein, Mis5 Protein, P105MCM Protein, Mcmd6 Protein, mis5 Protein, mmcm6 Protein, ASP-l1 Protein, D1Wsu22e Protein, Minichromosome maintenance 6 Protein, DNA replication licensing factor MCM6 Protein, minichromosome maintenance complex component 6 Protein, minichromosome maintenance complex component 6 L homeolog Protein, DNA replication licensing factor mcm-6 Protein, Mcm6 Protein, TP02_0113 Protein, PVX_114735 Protein, CMU_027970 Protein, MCM6 Protein, mcm6.L Protein, mcm-6 Protein

Sujet: DNA replication licensing factor MCM6 (EC 3.6.4.12) (Mis5 homolog),FUNCTION: Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. {ECO:0000269|PubMed:10567526}.

Poids moléculaire: 92.9 kDa

UniProt: P97311

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA