NEIL1 Protein (AA 2-389) (His tag)

N° du produit ABIN3136614

Détail du produit

Antigène: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-389

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette NEIL1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: PEGPELHLAS HFVNETCKGL VFGGCVEKSS VSRNPEVPFE SSAYHISALA RGKELRLTLS PLPGSQPPQK PLSLVFRFGM SGSFQLVPAE ALPRHAHLRF YTAPPAPRLA LCFVDIRRFG HWDPGGEWQP GRGPCVLLEY ERFRENVLRN LSDKAFDRPI CEALLDQRFF NGIGNYLRAE ILYRLKIPPF EKARTVLEAL QQCRPSPELT LSQKIKAKLQ NPDLLELCHL VPKEVVQLGG KGYGPERGEE DFAAFRAWLR CYGVPGMSSL RDRHGRTIWF QGDPGPLAPK GGRSQKKKSQ ETQLGAEDRK EDLPLSSKSV SRMRRARKHP PKRIAQQSEG AGLQQNQETP TAPEKGKRRG QRASTGHRRR PKTIPDTRPR EAGESSAS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Neil1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Autre désignation: Neil1 (NEIL1 Produits)

Synonymes: FPG1 Protein, NEI1 Protein, hFPG1 Protein, 2810450N13Rik Protein, Nei1 Protein, nei-like DNA glycosylase 1 Protein, nei like DNA glycosylase 1 Protein, nei endonuclease VIII-like 1 (E. coli) Protein, Neil1 Protein, NEIL1 Protein

Sujet: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC. {ECO:0000269|PubMed:12200441, ECO:0000269|PubMed:23434322}.

Poids moléculaire: 44.4 kDa Including tag.

UniProt: Q8K4Q6

Pathways: Réparation de l'ADN