NEIL1 Protein (His tag)

N° du produit ABIN7317344

Détail du produit

Antigène: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette NEIL1 protéine est marqué à la His tag.

Fonction: Recombinant Human NEIL1 Protein (His Tag)

Séquence: Met 1-Ser 390

Attributs du produit: A DNA sequence encoding the human NEIL1 (AAH10876.1) (Met 1-Ser 390) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.

Pureté: > 84 % as determined by reducing SDS-PAGE.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 8.0

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Autre désignation: NEIL1 (NEIL1 Produits)

Synonymes: FPG1 Protein, NEI1 Protein, hFPG1 Protein, 2810450N13Rik Protein, Nei1 Protein, nei-like DNA glycosylase 1 Protein, nei like DNA glycosylase 1 Protein, nei endonuclease VIII-like 1 (E. coli) Protein, Neil1 Protein, NEIL1 Protein

Sujet:

Background: NEIL1 is a member of DNA glycosylases. DNA glycosylases are a family homologous to the bacterial Fpg/Nei family. They play a role in base excision repair which is the mechanism by which damaged bases in DNA are removed and replaced. The first step of this process is catalyzed by DNA glycosylases. They remove the damaged nitrogenous base while leaving the sugar-phosphate backbone intact, creating an apurinic/apyrimidinic site, commonly referred to as an AP site. NEIL1 functions in base excision repair of DNA damaged by oxidation or by mutagenic agents. It acts as DNA glycosylase that recognizes and removes damaged bases. NEIL1 prefers to oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand and cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.

Synonym: FPG1,hFPG1,NEI1

Poids moléculaire: 45 kDa

Pathways: Réparation de l'ADN