Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A) (AA 19-352) (Active) Protéine

Détails pour le produit réf. ABIN4948749, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • WNT3A
  • Wnt-3a
  • vt
  • Zwnt[a]
  • wnt3
  • wnt3 l
  • wnt3l
  • wnt[a]
  • Xwnt-3a
  • wnt-3a
  • wnt3a-A
  • xwnt3a
  • WNT-3A
  • WNT3
  • wingless-type MMTV integration site family, member 3A
  • Wnt family member 3A
  • wingless-type MMTV integration site family member 3A L homeolog
  • WNT3A
  • Wnt3a
  • wnt3a
  • wnt3a.L
Attributs du protein
AA 19-352
14
3
1
1
1
1
1
Origine
Souris
13
6
1
1
1
1
1
1
1
Source
Mammalian Cells
11
7
6
1
1
Type de proteíne
Recombinant
Activité biologique
Active
Application
Functional Studies (Func)
Options
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N° du produit (Fournisseur)
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Specificité Stem cell or organoid culture
Purification This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.
Pureté 70-75 %
niveau d'endotoxine Less than 0.1 ng/μg (1 IEU/μg)
Biological Activity Comment Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 02DEC2015) generate 100- fold increase of luciferase activity compared to control (buffer without Wnt3a). EC50 is about 6 ng/mL.
Nom de la protéine
Sujet Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene
Poids moléculaire 41 kDa evaluated by SDS-PAGE under reducing conditions
ID gène 22416
NCBI Accession NC_000077
UniProt P27467
Pathways Signalisation WNT, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
Indications d'application 31-60 ng/mL
To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only
Restrictions For Research Use only
Format Liquid
Buffer Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA
Conseil sur la manipulation Mix the protein by pipetting up and down but not by vortexing
Stock -20 °C
Stockage commentaire Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.
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