WNT3A Protein (AA 19-352)

N° du produit ABIN4948749

Détail du produit

Antigène: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 19-352

Origine: Souris

Source: Mammalian Cells

Application: Functional Studies (Func)

Specificité: Stem cell or organoid culture

Purification: This protein was purified using a combination of ion exchange, affinity column with Wnt signaling inhibitor-bound sepharose beads, and followed by gel filtration.

Pureté: 70-75 %

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg)

Biological Activity Comment: Wnt3a activity has been measured using TCF-based Wnt reporter stable cell line. 10 ng/mL of Wnt3a (Lot: 02DEC2015) generate 100- fold increase of luciferase activity compared to control (buffer without Wnt3a). EC50 is about 6 ng/mL.

Information d'application

Indications d'application: 31-60 ng/mL
To treat cell lines, dilute the protein solution at least 200 times in medium, to treat stem cells, dilute the protein solution at least 500 times in medium. Diluted Wnt proteins in medium or phosphate buffer can be stored at 4 °C for few days only

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate buffer pH 7.4-7.6, 1 % CHAPS, 0.1 % BSA

Conseil sur la manipulation: Mix the protein by pipetting up and down but not by vortexing

Stock: -20 °C

Stockage commentaire: Keep the protein frozen until use. Refreeze aliquots at -20C or below but avoid freeze-thaw circles.

Détail du antigène

Antigène: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Autre désignation: Wnt3a (WNT3A Produits)

Sujet: Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene

Poids moléculaire: 41 kDa evaluated by SDS-PAGE under reducing conditions

ID gène: 22416

NCBI Accession: NC_000077

UniProt: P27467

Pathways: Signalisation WNT, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity