IGFBP3
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by SDS-PAGE and Coomassie blue staining
Func, AbP, PI, STD
Active
IGFBP3
(Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
Type de proteíne
Recombinant
Activité biologique
Active
Attributs du protein
Transcript Variant 2
Origine
Humain
Source
Escherichia coli (E. coli)
Application
Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
Specificité
Optimal preservation of protein structure, post-translational modifications and functions.
Attributs du produit
Recombinant human IGFBP3 (transcript variant 2) protein expressed in E. coli.
Produced with end-sequenced ORF clone
Tested for bioactivity.
Pureté
> 95 % as determined by SDS-PAGE and Coomassie blue staining
niveau d'endotoxine
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Biological Activity Comment
ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is less than or equal to 0.2 ug/ml in presence of 15 ng/ml of human IGF-II.
IGFBP3
Origine: Humain
Hôte: Escherichia coli (E. coli)
Recombinant
> 97 %
WB, SDS, Imm, PC
Indications d'application
Recombinant human proteins can be used for: Native antigens for optimized antibody production Positive controls in ELISA and other antibody assays Protein-protein interaction In vitro biochemical assays and cell-based functional assays
Restrictions
For Research Use only
Buffer
Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
Conseil sur la manipulation
Resuspend the protein in the desired concentration in proper buffer
Stock
-80 °C
Stockage commentaire
Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Antigène
IGFBP3
(Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.