Glycoprotein / GP (Virus) (partial) protein (His tag)

N° du produit ABIN5608204

Détail du produit

Antigène: Glycoprotein / GP (Virus)

Type de proteíne: Recombinant

Attributs du protein: partial

Origine: Ebola Virus, Bundibugyo Virus (BDBV)

Source: HEK-293 Cells

Purification/Conjugué: His tag

Application: Western Blotting (WB)

Séquence: Ile 33 - Arg 324

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Endotoxin level is less than 1.0 EU per ug by the LAL method.

Information d'application

Indications d'application: This recombinant protein can be used for WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C,-80 °C

Stockage commentaire: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.

Détail du antigène

Antigène: Glycoprotein / GP (Virus)

Classe de substances: Viral Protein

Sujet: EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160- kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

Poids moléculaire: 34.3 kDa

ID gène: 9487265

UniProt: B8XCN1