Glycoprotein / GP (Virus) (AA 33-637) protein (His tag)

N° du produit ABIN2870779

Détail du produit

Antigène: Glycoprotein / GP (Virus)

Type de proteíne: Recombinant

Attributs du protein: AA 33-637

Origine: Ebola Virus

Source: HEK-293 Cells

Purification/Conjugué: His tag

Séquence: AA 33-637

Attributs du produit: Ebolavirus EBOV(subtype Zaire,strain Kikwit-95) GP, containing GP1 and GP2, is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 67 kDa. The predicted N-terminus is Ile 33. The reducing (R) protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP1) in SDS-PAGE .

Pureté: >85 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Conseil sur la manipulation: Please avoid repeated freeze-thaw cycles.

Stock: -20 °C

Détail du antigène

Antigène: Glycoprotein / GP (Virus)

Classe de substances: Viral Protein

Sujet: EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160- kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

Poids moléculaire: 67.2 kDa