GAPDH
Origine: Souris
Hôte: Escherichia coli (E. coli)
Recombinant
> 97 %
SDS, WB, Imm, PC
Indications d'application
This recombinant protein can be used for WB.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
50 mM Tris, 150 mM NaCl, pH 7.5
Conseil sur la manipulation
Avoid repeated freeze-thaw cycles.
Stock
-20 °C,-80 °C
Stockage commentaire
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is also known as Peptidyl-cysteine S-nitrosylase GAPDH, which is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. GAPDH can participate in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Also, GAPDH can modulate the organization and assembly of the cytoskeleton. Furthermore, GAPDH is a component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.