VPS4B Protein (AA 1-444) (His tag)

N° du produit ABIN5853272

Détail du produit

Antigène: VPS4B (vps4b) (Vacuolar Protein Sorting 4 Homolog B (vps4b))

Type de proteíne: Recombinant

Attributs du protein: AA 1-444

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette VPS4B protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSHMSSTSP NLQKAIDLAS KAAQEDKAGN YEEALQLYQH AVQYFLHVVK YEAQGDKAKQ SIRAKCTEYL DRAEKLKEYL KNKEKKAQKP VKEGQPSPAD EKGNDSDGEG ESDDPEKKKL QNQLQGAIVI ERPNVKWSDV AGLEGAKEAL KEAVILPIKF PHLFTGKRTP WRGILLFGPP GTGKSYLAKA VATEANNSTF FSISSSDLVS KWLGESEKLV KNLFQLAREN KPSIIFIDEI DSLCGSRSEN ESEAARRIKT EFLVQMQGVG VDNDGILVLG ATNIPWVLDS AIRRRFEKRI YIPLPEPHAR AAMFKLHLGT TQNSLTEADF RELGRKTDGY SGADISIIVR DALMQPVRKV QSATHFKKVR GPSRADPNHL VDDLLTPCSP GDPGAIEMTW MDVPGDKLLE PVVSMSDMLR SLSNTKPTVN EHDLLKLKKF TEDFGQEG

Pureté: > 90 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 30 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: VPS4B (vps4b) (Vacuolar Protein Sorting 4 Homolog B (vps4b))

Autre désignation: VPS4B (vps4b Produits)

Synonymes: zgc:63682 Protein, vps4a Protein, SKD1 Protein, SKD1B Protein, VPS4-2 Protein, 8030489C12Rik Protein, Skd1 Protein, vacuolar protein sorting 4 homolog B Protein, vacuolar protein sorting 4 homolog B S homeolog Protein, vacuolar protein sorting 4b homolog B (S. cerevisiae) Protein, vacuolar protein sorting 4 homolog B (S. cerevisiae) Protein, vacuolar protein sorting 4B Protein, Vps4b Protein, vps4b.S Protein, vps4b Protein, VPS4B Protein

Sujet: Vacuolar protein sorting-associated protein 4B, also known as VPS4B, belongs to the AAA (ATPases associated with diverse cellular activities) family. It is involved in late steps of the endosomal multivesicular bodies (MVB) pathway and recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Dominant negative mutant of VPS4B inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding. Recombinant human VPS4B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 51.8 kDa (468aa)

NCBI Accession: NP_004860

UniProt: O75351

Pathways: Dynamique des Microtubules, CXCR4-mediated Signaling Events