BCCIP Protein (AA 1-314) (His tag)

N° du produit ABIN5853793

Détail du produit

Antigène: BCCIP (BRCA2 and CDKN1A Interacting Protein (BCCIP))

Type de proteíne: Recombinant

Attributs du protein: AA 1-314

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette BCCIP protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSEFMASRS KRRAVESGVP QPPDPPVQRD EEEEKEVENE DEDDDDSDKE KDEEDEVIDE EVNIEFEAYS LSDNDYDGIK KLLQQLFLKA PVNTAELTDL LIQQNHIGSV IKQTDVSEDS NDDMDEDEVF GFISLLNLTE RKGTQCVEQI QELVLRFCEK NCEKSMVEQL DKFLNDTTKP VGLLLSERFI NVPPQIALPM YQQLQKELAG AHRTNKPCGK CYFYLLISKT FVEAGKNNSK KKPSNKKKAA LMFANAEEEF FYEKAILKFN YSVQEESDTC LGGKWSFDDV PMTPLRTVML IPGDKMNEIM DKLKEYLSV

Pureté: > 90 % by SDS - PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1  mM DTT

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: BCCIP (BRCA2 and CDKN1A Interacting Protein (BCCIP))

Autre désignation: BCCIP (BCCIP Produits)

Synonymes: zgc:110272 Protein, mp:zf637-1-000774 Protein, BCCIP Protein, DKFZp459K2416 Protein, MGC82792 Protein, tok-1 Protein, MGC89311 Protein, TOK-1 Protein, TOK1 Protein, 1110013J05Rik Protein, BRCA2 and CDKN1A interacting protein Protein, BRCA2 and CDKN1A interacting protein L homeolog Protein, BCCIP Protein, bccip Protein, bccip.L Protein, Bccip Protein

Sujet: BCCIP was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Recombinant human BCCIP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 38.6kDa(339aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

NCBI Accession: NP_510868

UniProt: Q9P287