AKR1D1 Protein (AA 1-326)

N° du produit ABIN5854502

Détail du produit

Antigène: AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-326

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Séquence: MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY

Pureté: > 95 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.5) containing 1  mM DTT, 0.1M NaCl, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: AKR1D1 (Aldo-Keto Reductase Family 1, Member D1 (AKR1D1))

Autre désignation: AKR1D1 (AKR1D1 Produits)

Synonymes: 3o5bred Protein, CBAS2 Protein, SRD5B1 Protein, 3-oxo-5-beta-steroid 4-dehydrogenase-like Protein, aldo-keto reductase family 1 member D1 Protein, aldo-keto reductase family 1, member D1 Protein, aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) Protein, LOC491379 Protein, AKR1D1 Protein, Akr1d1 Protein

Sujet: AKR1D1 also known as 3-oxo-5beta-steroid 4-deghydrogenase isoform 1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-betareduction of bile acid intermediates and steroid hormones which carry a delta (4)-3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant AKR1D1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 37.3 kDa (326aa)

NCBI Accession: NP_005980

UniProt: P51857

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process