IL-2 Protein (AA 21-169) (His tag)
-
- Antigène Voir toutes IL-2 (IL2) Protéines
- IL-2 (IL2) (Interleukin 2 (IL2))
- Activité biologique
- Active
- Attributs du protein
- AA 21-169
-
Origine
- Souris
-
Source
- HEK-293 Cells
- Purification/Conjugué
- Cette IL-2 protéine est marqué à la His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Attributs du produit
- Tag location: N-terminal His Tag
- Pureté
- > 97 %
- Biological Activity Comment
- Interleukin-2 (IL-2), the main lymphokine produced by activated CD4+ T-helper lymphocytes, is a potent T-cell growth regulator. In addition to this activity, IL-2 was found to decrease the proliferation of 3T3 fibroblasts in time- and dose-dependent manner. Thus 3T3 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of IL2 for 48h, then 3T3 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. The inhibitory effect of IL-2 on cell proliferation of 3T3 cells observed by inverted microscope was shown in Figure 1.
- Top Product
- Discover our top product IL2 Protéine
-
Interleukin 2 (IL2) (AA 21-153) protein
IL2 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) (AA 21-169) protein (His-Avi Tag)IL2 Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) (AA 21-169) protein (His-Avi Tag,Biotin)IL2 Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) (Active) proteinIL2 Origine: Rat Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 2 (IL2) (AA 21-153) (Active) proteinIL2 Origine: Humain Hôte: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 2 (IL2) (Active) proteinIL2 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Interleukin 2 (IL2) (AA 21-153) (Active) protein (Fc Tag,AVI tag,Biotin)IL2 Origine: Humain Hôte: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 2 (IL2) protein (His tag)IL2 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >90 % as determined by reducing SDS-PAGE.
Interleukin 2 (IL2) protein (His tag)IL2 Origine: Rat Hôte: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 2 (IL2) (AA 1-153) protein (GST tag)IL2 Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
-
- Indications d'application
- Isoelectric Point: 4.9
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Agent conservateur
- Dithiothreitol (DTT), Other preservative, ProClin
- Précaution d'utilisation
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
- Antigène
- IL-2 (IL2) (Interleukin 2 (IL2))
- Abstract
- IL2 Produits
- Synonymes
- IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein
- Sujet
- Alternative Names: TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin
- Poids moléculaire
- 25kDa
- UniProt
- P04351
- Pathways
- Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
-
