IL17C Protein (AA 36-187) (His tag)

N° du produit ABIN6239767

Détail du produit

Antigène: IL17C (Interleukin 17C (IL17C))

Activité biologique: Active

Attributs du protein: AA 36-187

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette IL17C protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 95 %

Biological Activity Comment: IL17C (Interleukin-17C) is a T cell-derived cytokine that shares the sequence similarity with IL17. IL17C is thought to plays a crucial role in innate immunity of the epithelium. IL17C binds to IL17RA with affinity. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17C with recombinant human IL17RA. Briefly, IL17C were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17C with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of IL17C with IL17RA.

Information d'application

Indications d'application: Isoelectric Point: 8.6

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: IL17C (Interleukin 17C (IL17C))

Abstract: IL17C Produits

Synonymes: IL17C Protein, il17-3 Protein, CX2 Protein, IL-17C Protein, IL-21 Protein, interleukin 17C Protein, interleukin 17c Protein, IL17C Protein, il17c Protein, Il17c Protein

Sujet: Alternative Names: Cytokine CX2

Poids moléculaire: 19kDa

UniProt: Q9P0M4

Pathways: Cellular Response to Molecule of Bacterial Origin