VEGFC Protein (AA 47-413) (His tag)

N° du produit ABIN6239783

Détail du produit

Antigène: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Activité biologique: Active

Attributs du protein: AA 47-413

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette VEGFC protéine est marqué à la His tag.

Application: Cell Culture (CC), Activity Assay (AcA)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 90 %

Biological Activity Comment: Vascular endothelial growth factor C (VEGFC) is a protein that is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It plays key roles in the physiology and pathology of many aspects of the cardiovascular system, including vasculogenesis, hematopoiesis, angiogenesis and vascular permeability. To test the effect of VEGFC on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFC. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. The dose-effect curve of VEGFC was shown in Figure 1. It was obvious that VEGFC significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 0.304 to 1.339μg/mL The dose-effect curve of VEGFC on ECV304 cells

Information d'application

Indications d'application: Isoelectric Point: 8.2

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Abstract: VEGFC Produits

Synonymes: VEGFC Protein, vegf-c Protein, xvegf-c Protein, Flt4-L Protein, VRP Protein, AW228853 Protein, VEGF-C Protein, vascular endothelial growth factor c Protein, vascular endothelial growth factor C Protein, vascular endothelial growth factor C S homeolog Protein, vegfc Protein, VEGFC Protein, vegfc.S Protein, Vegfc Protein

Sujet: Alternative Names: VEGF-C, Flt4-L, VRPL, Vascular Endothelial Growth Factor-Related Protein

Poids moléculaire: 45kDa

UniProt: P49767

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2