Caspase 1 Protein (CASP1) (AA 119-296) (His tag)
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- Antigène Voir toutes Caspase 1 (CASP1) Protéines
- Caspase 1 (CASP1)
- Activité biologique
- Active
- Attributs du protein
- AA 119-296
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Origine
- Rat
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette Caspase 1 protéine est marqué à la His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Attributs du produit
- Tag location: N-terminal His Tag
- Pureté
- > 95 %
- Biological Activity Comment
- Caspase 1 (CASP1) cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. It also has lots of other function, such as defenses against pathogens, cleaves and activates sterol regulatory element binding proteins (SREBPs), promotes apoptosis. Besides, Interleukin 1 Alpha (IL1a) has been identified as an interactor of CASP1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat CASP1 and recombinant rat IL1a. Briefly, CASP1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CASP1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of CASP1 and IL1a was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CASP1 with IL1a.
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- Discover our top product CASP1 Protéine
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- Indications d'application
- Isoelectric Point: 7.7
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Agent conservateur
- Dithiothreitol (DTT), Other preservative, ProClin
- Précaution d'utilisation
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Antigène
- Caspase 1 (CASP1)
- Abstract
- CASP1 Produits
- Synonymes
- ICE Protein, IL1BC Protein, P45 Protein, Il1bc Protein, Ice Protein, CASP1 Protein, CG5370 Protein, DCP-1 Protein, DCP1 Protein, Dcp1 Protein, Dmel\\CG5370 Protein, ccp1 Protein, dcp-1 Protein, dcp1 Protein, l(2)01862 Protein, l(2)02132 Protein, caspase 1 Protein, caspase 1, apoptosis-related cysteine peptidase Protein, caspase 4, apoptosis-related cysteine peptidase Protein, Death caspase-1 Protein, caspase-1 Protein, caspase Protein, caspase recruitment domain-containing protein 16 Protein, caspase 4 Protein, CASP1 Protein, Casp1 Protein, CASP4 Protein, Dcp-1 Protein, Caspase1 Protein, LOC5564196 Protein, LOC451519 Protein
- Sujet
- Alternative Names: ICE, IL1BC, IL1-BC, P45, Interleukin 1 Beta Converting Enzyme, Apoptosis-Related Cysteine Peptidase, Interleukin-1 beta convertase
- Poids moléculaire
- 21kDa
- UniProt
- P43527
- Pathways
- Apoptose, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome
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