ESM1 Protein (AA 20-184) (His tag)

N° du produit ABIN6239900

Détail du produit

Antigène: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Activité biologique: Active

Attributs du protein: AA 20-184

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette ESM1 protéine est marqué à la His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Attributs du produit: Tag location: N-terminal His Tag

Pureté: > 90 %

Biological Activity Comment: Endothelial cell-specific molecule 1 (ESM1) is a proteoglycan secreted by endothelial cells (primarily in the human lung and kidney tissues) and its mRNA expression is regulated by inflammatory cytokines. Endocan expression, which detected in various epithelia and adipocytes has been shown to be upregulated by vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF-2), TNF alpha, IL1 beta, or lipopolysaccharide and downregulated by IFN gamma. Genetically engineered cells overexpressing ESM1 induce tumor formation, implying that ESM1 might be involved in the pathophysiology of tumor growth in vivo. Besides, Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) has been identified as an interactor of ESM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ESM1 and recombinant human LFA1a. Briefly, ESM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LFA1a-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti- ESM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of ESM1 and LFA1a was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ESM1 with LFA1a.

Information d'application

Indications d'application: Isoelectric Point: 6.7

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Agent conservateur: Dithiothreitol (DTT), Other preservative, ProClin

Précaution d'utilisation: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Détail du antigène

Antigène: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Autre désignation: Endothelial Cell Specific Molecule 1 (ESM1) (ESM1 Produits)

Synonymes: ESM1 Protein, zgc:153027 Protein, 0610042H23Rik Protein, AV004503 Protein, ESM-1 Protein, Pg25 Protein, endocan Protein, endothelial cell specific molecule 1 Protein, endothelial cell-specific molecule 1 Protein, ESM1 Protein, esm1 Protein, Esm1 Protein

Sujet: Alternative Names: Endocan

Poids moléculaire: 19kDa

UniProt: Q9NQ30

Pathways: Growth Factor Binding