MAP4K3 Protein (AA 1-321) (His tag)

N° du produit ABIN6387691

Détail du produit

Antigène: MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-321

Origine: E. coli

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette MAP4K3 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL

Pureté: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))

Autre désignation: glk (MAP4K3 Produits)

Synonymes: GLK Protein, MAPKKKK3 Protein, MEKKK 3 Protein, MEKKK3 Protein, RAB8IPL1 Protein, 4833416M01Rik Protein, 4833416M07Rik Protein, 9530052P13Rik Protein, Glk Protein, mitogen-activated protein kinase kinase kinase kinase 3 Protein, MAP4K3 Protein, Map4k3 Protein

Sujet: Glk belongs to the bacterial glucokinase family. This protein is not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E.coli glk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Poids moléculaire: 37.1kDa (344aa), confirmed by MALDI-TOF

NCBI Accession: NP_416889

UniProt: P0A6V8

Pathways: Signalisation MAPK