CA1 Protein (AA 1-261) (His tag)

N° du produit ABIN5778417

Détail du produit

Antigène: CA1 (Carbonic Anhydrase I (CA1))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 1-261

Origine: Humain

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette CA1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F

Pureté: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 10 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: CA1 (Carbonic Anhydrase I (CA1))

Autre désignation: Carbonic anhydrase 1 (CA1 Produits)

Synonymes: CA-I Protein, CAB Protein, Car1 Protein, AW555628 Protein, Ca1 Protein, Car-1 Protein, BG:DS00941.1 Protein, CAH Protein, CG7820 Protein, Dmel\\CG7820 Protein, Gh7 Protein, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 Protein, ATBCA1 Protein, ATSABP3 Protein, BETA CARBONIC ANHYDRASE 1 Protein, F4P13.5 Protein, F4P13_5 Protein, SABP3 Protein, SALICYLIC ACID-BINDING PROTEIN 3 Protein, carbonic anhydrase 1 Protein, CA1 Protein, LOC100135826 Protein, GB15888 Protein, carbonic anhydrase 1 Protein, Carbonic anhydrase 1 Protein, carbonic anhydrase I Protein, carbonic anhydrase Protein, CA1 Protein, Car1 Protein, CAH1 Protein, LOC100135826 Protein, LOC408827 Protein, Tsp_05114 Protein, LOC100153915 Protein, LOC100050192 Protein, Ca1 Protein

Sujet: Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O <-> HCO3- + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques.

Poids moléculaire: 31.0 kDa (281aa), confirmed by MALDI-TOF

NCBI Accession: NP_001122301

UniProt: P00915