Lactate Dehydrogenase A Protein (LDHA) (AA 1-332) (His tag)
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- Antigène Voir toutes Lactate Dehydrogenase A (LDHA) Protéines
- Lactate Dehydrogenase A (LDHA)
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 1-332
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Origine
- Rat
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Source
- Baculovirus infected Insect Cells
- Purification/Conjugué
- Cette Lactate Dehydrogenase A protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH
- Pureté
- > 95 % by SDS - PAGE
- niveau d'endotoxine
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product LDHA Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 1 mM DTT, 20 % glycerol.
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- Lactate Dehydrogenase A (LDHA)
- Autre désignation
- Ldha (LDHA Produits)
- Synonymes
- GSD11 Protein, LDH1 Protein, LDHM Protein, LDH-M Protein, ldh-h Protein, ldha Protein, ldha-B Protein, ldhbb Protein, trg-5 Protein, Ldh1 Protein, Ldhm Protein, l7R2 Protein, gsd11 Protein, ldh-m Protein, ldh1 Protein, ldhm Protein, pig19 Protein, ldha1 Protein, ldhab Protein, LDH-A Protein, LDHC Protein, lactate dehydrogenase A Protein, lactate dehydrogenase B L homeolog Protein, lactate dehydrogenase A4 Protein, lactate dehydrogenase A L homeolog Protein, L-lactate dehydrogenase A chain Protein, LDHA Protein, ldhb.L Protein, Ldha Protein, ldha Protein, ldha.L Protein, LOC100713875 Protein
- Sujet
- Ldha, also known as lactate dehydrogenase A, catalyzes the conversion of pyruvate to lactate, utilizing NADH as a cofactor. It has been identified as a potential therapeutic target in the area of cancer metabolism. Reduction in Ldha activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Recombinant rat Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Poids moléculaire
- 37.5kDa (340aa) 28-40KDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_058721
- UniProt
- P04642
- Pathways
- L'effet Warburg
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