ALPL Protein (AA 19-503) (His tag)

N° du produit ABIN6387919

Détail du produit

Antigène: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: AA 19-503

Origine: Souris

Source: Baculovirus infected Insect Cells

Purification/Conjugué: Cette ALPL protéine est marqué à la His tag.

Application: SDS-PAGE (SDS)

Séquence: VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVT AARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTR CNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQL MHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLAL DPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQA LHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNG PGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYAS CIGANLDHCA WAGSGLEHHH HHH

Pureté: > 95 % by SDS - PAGE

niveau d'endotoxine: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 46,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Bioactivity Validated

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Autre désignation: Alpl (ALPL Produits)

Synonymes: ALP Protein, iap Protein, alpl Protein, AP-TNAP Protein, APTNAP Protein, HOPS Protein, TNAP Protein, TNSALP Protein, Akp-2 Protein, Akp2 Protein, PHOA Protein, TNS-AP Protein, alkaline phosphatase, liver/bone/kidney L homeolog Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase, intestinal, gene 2 Protein, alkaline phosphatase, liver/bone/kidney pseudogene Protein, alpl.L Protein, ALPL Protein, alpi.2 Protein, LOC100031702 Protein, Alpl Protein

Sujet: Alpl, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific). The product of this gene is a membrane-bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. A proposed function of this form of the enzyme is matrix mineralization. Recombinant mouse Alpl, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Poids moléculaire: 54.5kDa (493aa) 50-70KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_031457

UniProt: P09242