EPOR Protein (AA 25-250) (His tag)
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- Antigène Voir toutes EPOR Protéines
- EPOR (Erythropoietin Receptor (EPOR))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 25-250
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Origine
- Humain
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Source
- Baculovirus infected Insect Cells
- Purification/Conjugué
- Cette EPOR protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDPHHHH HH
- Pureté
- > 95 % by SDS - PAGE
- niveau d'endotoxine
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured by its ability to inhibit EPO dependent proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect less or equal to 70ng/ml.
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- Discover our top product EPOR Protéine
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Erythropoietin Receptor (EPOR) (AA 25-138) protein (His-IF2DI Tag)
EPOR Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Erythropoietin Receptor (EPOR) (AA 1-508) protein (GST tag)EPOR Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
Erythropoietin Receptor (EPOR) (AA 31-130) protein (GST tag)EPOR Origine: Humain Hôte: Wheat germ Recombinant WB, ELISA, AA, AP
Erythropoietin Receptor (EPOR) (Active) protein (His tag)EPOR Origine: Souris Hôte: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Antigène
- EPOR (Erythropoietin Receptor (EPOR))
- Autre désignation
- EPOR (EPOR Produits)
- Synonymes
- EPOR Protein, epor Protein, EPO-R Protein, erythropoietin receptor Protein, erythropoietin receptor L homeolog Protein, EPOR Protein, epor Protein, Epor Protein, epor.L Protein
- Sujet
- EPOR, also known as Erythropoietin receptor, mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, it dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. So it may also activate the LYN tyrosine kinase. Recombinant human EPOR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Poids moléculaire
- 25.6kDa (232aa) 28-40kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_000112
- UniProt
- P19235
- Pathways
- Signalistation JAK/STAT
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