ADK Protein (AA 1-361) (His tag)
-
- Antigène Voir toutes ADK Protéines
- ADK (Adenosine Kinase (ADK))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
- Attributs du protein
- AA 1-361
-
Origine
- Humain
-
Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette ADK protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MGSMAAADEP KPKKLKVEAP QALSENVLFG MGNPLLDISA VVDKDFLDKY SLKPNDQILA EDKHKELFDE LVKKFKVEYH AGGSTQNSMK VAQWLIQEPH KAATFFGCIG IDKFGEILKR KAADAHVDAH YYEQNEQPTG TCAACITGGN RSLVANLAAA NCYKKEKHLD LERNWVLVEK ARVYYIAGFF LTVSPESVLK VARYAAENNR VFTLNLSAPF ISQFFKEALM DVMPYVDILF GNETEAATFA REQGFETKDI KEIAKKAQAL PKVNSKRQRT VIFTQGRDDT IVAAENDVTA FPVLDQNQEE IIDTNGAGDA FVGGFLSQLV SDKPLTECIR AGHYAASVII RRTGCTFPEK PDFH
- Pureté
- > 95 % by SDS - PAGE
- niveau d'endotoxine
- < 1.0 EU per 1 ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 100 pmol/min/ and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.
- Top Product
- Discover our top product ADK Protéine
-
-
- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
-
Bioactivity Validated
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.0) containing 20 % glycerol, 50 mM NaCl, 1 mM EDTA
- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Antigène
- ADK (Adenosine Kinase (ADK))
- Autre désignation
- Adk (ADK Produits)
- Synonymes
- AK Protein, ADK Protein, DDBDRAFT_0186795 Protein, DDBDRAFT_0230174 Protein, DDB_0186795 Protein, DDB_0230174 Protein, ATPADK1 Protein, F3G5.4 Protein, F3G5_4 Protein, adenosine kinase Protein, adk Protein, 2310026J05Rik Protein, 5033405D03Rik Protein, AI255373 Protein, AI987814 Protein, Ak Protein, adenosine kinase Protein, ATP:adenosine 5'-phosphotransferase Protein, Adenosine kinase Protein, adenosine kinase S homeolog Protein, ADK Protein, adk Protein, Adk Protein, CNN02130 Protein, LOC5572668 Protein, CMU_026370 Protein, adk.S Protein
- Sujet
- Adk, also known as Adenosine kinase, is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant mouse Adk, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 42.5 kDa (384aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_598840
- UniProt
- P55264
- Pathways
- Ribonucleoside Biosynthetic Process
-