TNF alpha Protéine

N° du produit ABIN6700748

N° du produit (Fournisseur): 010-001-321

Cette protéine Recombinant TNF alpha est exprimée dans Escherichia coli (E. coli) et a été mentionnée dans 1 publication.

Aperçu rapide pour TNF alpha Protéine (ABIN6700748)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Origine: Souris

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 98,00%

Détail du produit

N° du produit (Fournisseur): 010-001-321

Fournisseur: Rockland

Fonction: TNF-α Mouse Recombinant Protein

Purification: Purity was determined to be greater than 98% by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE.

niveau d'endotoxine: Measured by LAL is 0.04 EU/μg protein (with 50% confidence range).

Biological Activity Comment: Recombinant Mouse TNFα is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is 13-20 pg/mL.

Information d'application

Indications d'application:

Application Note: TNFα protein has been tested by SDDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-TNFα in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 20 μL (20-200 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Référence

Sun, Yau, Wong, Li, Huang, Yao: "Role of TRPM2 in H(2)O(2)-induced cell apoptosis in endothelial cells." dans: PloS one, Vol. 7, Issue 8, pp. e43186, (2013) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tnf

Sujet:

Synonyms: Tumor necrosis factor, TNF-alpha, TNF-a, TNFα, cytokine, Tumor necrosis factor ligand superfamily member 2, Cachectin

Background: Tumor Necrosis Factor-alpha (TNF-a) is pleiotrophic cytokine secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following stimulation by bacterial LPS . TNF-a activity is mediated via interactions with TNFR1 &TNFR2 receptors. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2 & GM-CSF. Recombinant mouse TNF-a produced in E.Coli is a non-glycosylated polypeptide chain containing 156 amino acids and having a molecular mass of 17,300 daltons.

UniProt: P06804

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome