TNF alpha Protéine

N° du produit ABIN6700750

N° du produit (Fournisseur): 009-f01-w31-0050

Cette protéine Recombinant TNF alpha est produite dans Escherichia coli (E. coli).

Aperçu rapide pour TNF alpha Protéine (ABIN6700750)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: 96,00%

Classe de qualité: Animal-Free

Détail du produit

N° du produit (Fournisseur): 009-f01-w31-0050

Fournisseur: Rockland

Fonction: Human Tumor Necrosis Factor alpha Recombinant Protein (Animal Free)

Purification: Tumor Necrosis Factor alpha is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 96% as determined by reducing and non-reducing SDS-PAGE.

niveau d'endotoxine: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D and is typically less than 0.05 ng/mL.

Information d'application

Indications d'application:

Application Note: Tumor Necrosis Factor alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Tumor Necrosis Factor alpha in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 50μL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Date de péremption: 6 months

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF

Sujet:

Synonyms: TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2

Background: Tumor Necrosis Factor alpha (TNFα) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNFα activates signals through two receptors, TNFR1, which is expressed on most cell types, and TNFR2, which is expressed mainly on immune cells. TNFα can have many functions including, to stimulate of phagocytosis in macrophages, to chemoattract neutrophils, to increase insulin resistance and to induce fever. Recombinant human TNF-α is a non-glycosylated protein, containing 158 amino acids, with a molecular weight of 17.5 kDa.

UniProt: P01375

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome