Human ACE2, Fc Tag (MALS verified) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1). Predicted N-terminus: Gln 18 This protein carries a human IgG1 Fc tag at the C-terminus.
Pureté
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
ACE2
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
Indications d'application
The protein has a calculated MW of 110.0 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
50 mM Tris, 150 mM NaCl, Arginine, pH 7.5
Conseil sur la manipulation
Please avoid repeated freeze-thaw cycles.
Stock
-80 °C
Stockage commentaire
The product MUST be stored at -70°C or lower upon receipt -70°C for 3 months under sterile conditions.
Huang, Zhou, He, Chiem, Mabrouk, Nissly, Bird, Strauss, Sambhara, Ortega, Wohlfert, Martinez-Sobrido, Kuchipudi, Davidson, Lovell: "SARS-CoV-2 RBD Neutralizing Antibody Induction is Enhanced by Particulate Vaccination." dans: Advanced materials (Deerfield Beach, Fla.), Vol. 32, Issue 50, pp. e2005637, (2021) (PubMed).
Wei, Duan, Liu, Wang, Ding, Chen, Xie, Tian, Yu, Ge, Zhang, Chen, Li, Meng: "Chicken Egg Yolk Antibodies (IgYs) block the binding of multiple SARS-CoV-2 spike protein variants to human ACE2." dans: International immunopharmacology, Vol. 90, pp. 107172, (2021) (PubMed).
Leach, Ilca, Akbar, Ferrari, Bentley, Mattiuzzo, Onuoha, Miller, Ali, Rabbitts: "A tetrameric ACE2 protein broadly neutralizes SARS-CoV-2 spike variants of concern with elevated potency." dans: Antiviral research, Vol. 194, pp. 105147, (2021) (PubMed).
Guo, Yao, Yang, Zhang, Qi, Gou, Xi, Liu, Zhang, Cheng, Wang, Rong, Chen, Kong: "Plasma-activated water: An alternative disinfectant for S protein inactivation to prevent SARS-CoV-2 infection." dans: Chemical engineering journal (Lausanne, Switzerland : 1996), pp. 127742, (2020) (PubMed).
ACE1 Protein, CD143 Protein, DCP Protein, DCP1 Protein, ICH Protein, MVCD3 Protein, ACEH Protein, 2010305L05Rik Protein, zgc:92514 Protein, angiotensin I converting enzyme Protein, angiotensin I converting enzyme 2 Protein, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 Protein, ACE Protein, ACE2 Protein, Ace2 Protein, ace2 Protein
Sujet
Angiotensin-converting enzyme 2 (ACE2) is also known as ACEH (ACE homolog), is an integral membrane protein with considerable homologous to ACE, which belongs to the peptidase M2 family. ACE2 is an exopeptidase that catalyses the conversion of angiotensin I to the nonapeptide angiotensin, or the conversion of angiotensin II to angiotensin 1-7. ACE2 may be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, ACE-2 serve as functional receptor for the spike glycoprotein of both coronaviruses. ACE2 is activated by chloride and fluoride, but not bromide and Inhibited by MLN-4760, cFP_Leu, and EDTA, but not by the ACE inhibitors linosipril, captopril and enalaprilat. ACE2 is active from pH 6 to 9, and the optimum pH is 6.5 in the presence of 1 M NaCl.