SARS-CoV-2 Spike Protein (P.1 - gamma, Trimer) (His tag,AVI tag,Biotin)

N° du produit ABIN7013289

Détail du produit

Antigène: SARS-CoV-2 Spike

Type de proteíne: Recombinant

Activité biologique: Active

Attributs du protein: P.1 - gamma, Trimer

Origine: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma

Source: HEK-293 Cells

Purification/Conjugué: Cette SARS-CoV-2 Spike protéine est marqué à la His tag,AVI tag,Biotin.

Fonction: Biotinylated SARS-CoV-2 S protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) trimer, His,Avitag™ (MALS verified)

Séquence: AA 16-1213

Specificité: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Attributs du produit: Biotinylated SARS-CoV-2 S protein trimer, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P/ A892P/ A899P/ A942P/ K986P/ V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. L18F/ T20N/ P26S/ D138Y/ R190S/ K417T/ E484K/ N501Y/ D614G/ H655Y/ T1027I/ V1176F) mutations were identified on the spike protein in the SARS-CoV-2 variant (known as P.1) which emerged in Brazil.

Pureté: >95 % as determined by SDS-PAGE.

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Commentaires:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS

Stock: -20 °C

Détail du antigène

Antigène: SARS-CoV-2 Spike

Autre désignation: SARS-CoV-2 S protein (SARS-CoV-2 Spike Produits)

Synonymes: E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

Classe de substances: Viral Protein

Sujet: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Poids moléculaire: 139.8 kDa