GUCY2D Protein (His-Avi Tag,Biotin)

N° du produit ABIN7199197

Cette protéine Recombinant GUCY2D est produite dans HEK-293 Cells.

Aperçu rapide pour GUCY2D Protein (His-Avi Tag,Biotin) (ABIN7199197)

Antigène: GUCY2D (Guanylate Cyclase 2D, Membrane (Retina-Specific) (GUCY2D))

Type de proteíne: Recombinant

Origine: Humain

Source: HEK-293 Cells

Pureté: >95 % as determined by SDS-PAGE.

Détail du produit

Purification/Conjugué: Cette GUCY2D protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Human GUCY2D / Retinal guanylyl cyclase 1 Protein, His,Avitag™

Séquence: Ala 52 - Glu 462

Attributs du produit: Biotinylated Human GUCY2D, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Ala 52 - Glu 462 (Accession # Q02846-1).

niveau d'endotoxine: Less than 1.0 EU per μg by the LAL method.

Information d'application

Indications d'application: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 46.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition due to glycosylation.

Commentaires:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: PBS, pH 7.4

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: GUCY2D (Guanylate Cyclase 2D, Membrane (Retina-Specific) (GUCY2D))

Autre désignation: GUCY2D / Retinal guanylyl cyclase 1

Sujet: Synonyms: GUCY2D,Retinal guanylyl cyclase 1,RETGC-1,CORD6,GUC1A4,GUC2D,ROS-GC,
GUCY2D also known as CORD6, GUC1A4, GUC2D ROS-GC and RETGC-1, belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Catalyzes the synthesis of cyclic GMP (cGMP) in rods and cones of photoreceptors. Plays an essential role in phototransduction, by mediating cGMP replenishment. May also participate in the trafficking of membrane-asociated proteins to the photoreceptor outer segment membrane.

Poids moléculaire: 46.7 kDa

NCBI Accession: NP_000171

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction