DLL1 Protein (His tag)

N° du produit ABIN7320250

Détail du produit

Antigène: DLL1 (delta-Like 1 (DLL1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Souris

Source: HEK-293 Cells

Purification/Conjugué: Cette DLL1 protéine est marqué à la His tag.

Fonction: Recombinant Mouse DLL1/Delta-1 Protein (His Tag)(Active)

Séquence: Met 1-Gln 516

Attributs du produit: A DNA sequence encoding the extracellular domain of mouse DLL1 (NP_031891.2) (Met 1-Gln 516) was expressed, with a polyhistidine tag at the C-terminus.

Pureté: > 95 % as determined by SDS-PAGE

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its ability to bind human NOTCH1 in a functional ELISA.2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 μg/mL.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4, 20 % glycerol

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: DLL1 (delta-Like 1 (DLL1))

Autre désignation: DLL1/Delta-1 (DLL1 Produits)

Synonymes: X-delta-1 Protein, XDelta1 Protein, Xdelta-1 Protein, delta Protein, delta-1 Protein, delta1 Protein, x-delta Protein, DELTA1 Protein, DL1 Protein, Delta Protein, DLK Protein, DLK-1 Protein, Delta1 Protein, FA1 Protein, PREF1 Protein, Pref-1 Protein, ZOG Protein, pG2 Protein, DLL1 Protein, AW742678 Protein, DlkI Protein, Ly107 Protein, Peg9 Protein, SCP1 Protein, pref-1 Protein, delta-like 1 Protein, delta like canonical Notch ligand 1 Protein, delta like non-canonical Notch ligand 1 Protein, delta-like 1 L homeolog Protein, delta-like 1 (Drosophila) Protein, delta-like 1 homolog (Drosophila) Protein, dll1 Protein, DLL1 Protein, DLK1 Protein, Dll1 Protein, dll1.L Protein, Dlk1 Protein

Sujet:

Background: Delta-like protein 1(DLL1), also known as Delta1, a single-pass type I membrane protein which contains one DSL domain and eight EGF-like domains,  acts as a ligand for Notch receptors, and positively regulates T-cell development. DLL1 is proteolytically processed in a similar manner to the Notch receptor, and it has been speculated to participate in bidirectional signaling. The proteolytic processing of DLL1 helps achieve an asymmetry in Notch signaling in initially equivalent myogenic cells and helps sustain the balance between differentiation and self-renewal. Interactions between DLL1 and Notch in trans activate the Notch pathway, whereas DLL1 binding to Notch in cis inhibits Notch signaling. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10. It had been demonstrated that DLL1 represents a substrate for several other members of the ADAM family. In co-transfected cells, DLL1 is constitutively cleaved by ADAM12, and the N-terminal fragment of DLL1 is released to medium. ADAM12-mediated cleavage of DLL1 is cell density-dependent, takes place in cis orientation, and does not require the presence of the cytoplasmic domain of ADAM12. Full-length DLL1, but not its N- or C-terminal proteolytic fragment, co-immunoprecipitates with ADAM12. By using a Notch reporter construct, we show that DLL1 processing by ADAM12 increases Notch signaling in a cell-autonomous manner. Furthermore, ADAM9 and ADAM17 have the ability to process DLL1. In contrast, ADAM15 does not cleave DLL1, although the two proteins still co-immunoprecipitate with each other. During fetal development, DLL1 is an essential Notch ligand in the vascular endothelium of large arteries to activate Notch1 and maintain arterial identity. DLL1-Notch signaling was required for VEGF receptor expression in fetal arteries.

Synonym: Delta1

Poids moléculaire: 55 kDa

NCBI Accession: NP_031891

Pathways: Signalisation Notch, Stem Cell Maintenance