HRG Protein (His tag)

N° du produit ABIN7317748

Détail du produit

Antigène: HRG (Histidine-Rich Glycoprotein (HRG))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette HRG protéine est marqué à la His tag.

Fonction: Recombinant Human HPRG/HRG Protein (His Tag)(Active)

Séquence: Met 1-Lys 525

Attributs du produit: A DNA sequence encoding the human HPRG (NP_000403.1) (Met 1-Lys 525) with a C-terminal polyhistidine tag was expressed.

Pureté: > 97 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. (Lamb-Wharton, R.J. and W.T. Morgen, 1993, Cell Immunol. 152: 544). Human HPRG immobilized at 1 μg/ml (100 μl/well) will induce >65% MOLT4 cell adhesion (1 x 10E5 cells/well) in the presence of 7.5 μg/ml Concanvalin A.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: HRG (Histidine-Rich Glycoprotein (HRG))

Autre désignation: HPRG/HRG (HRG Produits)

Synonymes: AI265597 Protein, AW413091 Protein, D16jh2 Protein, D18020 Protein, Hprg Protein, Hrgp Protein, HPRG Protein, HRGP Protein, THPH11 Protein, HRG1 Protein, histidine rich glycoprotein Protein, histidine-rich glycoprotein Protein, HRG Protein, LOAG_12427 Protein, LOC100597044 Protein, Hrg Protein

Sujet:

Background: Histidine-rich glycoprotein, also known as HRG and HPRG, is a glycoprotein located in plasma and platelets, and contains an unusually large amount of histidine and proline. In human, five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin-like modules (aa 19 - 254) and one His-Pro-rich region (aa 350 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear, but it is known that the protein binds heme, dyes and divalent metal ions. It inhibits rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. HPRG is evolutionarily, functionally and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated, at least partially, through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells.

Synonym: HPRG,HRGP,THPH11

Poids moléculaire: 59 kDa

NCBI Accession: NP_000403