TNF alpha Protéine

N° du produit ABIN7321256

Cette protéine Recombinant TNF alpha est produite dans Escherichia coli (E. coli).

Aperçu rapide pour TNF alpha Protéine (ABIN7321256)

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Rat

Source: Escherichia coli (E. coli)

Pureté: > 93 % as determined by SDS-PAGE

Détail du produit

Fonction: Recombinant Rat TNF-alpha/TNFA Protein (Active)

Séquence: Leu 80-Leu 235

Attributs du produit: A DNA sequence encoding the mature form of rat TNFα (P16599) (Leu 80-Leu 235) was expressed and purified, with an initial Met at the N-terminus.

Biological Activity Comment: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 20-80 pg/ml.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNF-alpha/TNFA

Sujet:

Background: Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1, CD120a, p55/60) and TNF-R2 (TNF receptor type 2, CD120b, p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Synonym: Tnf,MGC124630,RATTNF,TNF-alpha,Tnfa

Poids moléculaire: 17.4 kDa

UniProt: P16599

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome