SIRPA Protein (Biotin,His-Avi Tag)

N° du produit ABIN7319781

Détail du produit

Antigène: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Human Cells

Purification/Conjugué: Cette SIRPA protéine est marqué à la Biotin,His-Avi Tag.

Fonction: Recombinant Human Signal-Regulatory Protein alpha-1/SIRPA/CD172a (C-6His-Avi) Biotinylated

Séquence: Glu31-Arg370

Attributs du produit: Biotinylated Recombinant Human Signal-Regulatory Protein Alpha 1 is produced by our Mammalian expression system and the target gene encoding Glu31-Arg370 is expressed with a 6His, Avi tag at the C-terminus.

Pureté: >95 % as determined by reducing SDS-PAGE.

niveau d'endotoxine: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human SIRPA-His-Avi(Cat#CY28) at 10μg/ml (100 μl/well) can bind Human CD47-Fc(Cat#CG18). The ED50 of Human CD47-Fc(Cat#CG18) is 898 ng/ml.

Information d'application

Commentaires:

50-80 kDa

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Détail du antigène

Antigène: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Autre désignation: CD172a (SIRPA Produits)

Synonymes: BIT Protein, CD172A Protein, MFR Protein, MYD-1 Protein, P84 Protein, PTPNS1 Protein, SHPS1 Protein, SIRP Protein, SIRPA Protein, SIRP-A1 Protein, SIRP-B1 Protein, AI835480 Protein, Bit Protein, CD172a Protein, Ptpns1 Protein, SHP-1 Protein, SHPS-1 Protein, swc3 Protein, signal regulatory protein alpha Protein, myeloperoxidase Protein, signal-regulatory protein alpha Protein, tyrosine-protein phosphatase non-receptor type substrate 1 Protein, SIRPA Protein, MPO Protein, LOC458039 Protein, LOC100400378 Protein, LOC100590224 Protein, Sirpa Protein

Sujet:

Background: Signal Regulatory Protein α (SIRPα) is a monomeric approximately 90 kD type I transmembrane glycoprotein. The 504 amino acid human SIRPα contains two Ig-like C1-type domains and one Ig-like V-type domain. SIRPα can express in various tissues, mainly on brain and myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells. It also can detect in neurons, smooth muscle and endothelial cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPα acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPα shows adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. SIRPα engagement generally produces a negative regulatory signal, it may mediate negative regulation of phagocytosis, mast cell activation and dendritic cell activation.

Synonym: Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1, SHP Substrate 1, SHPS-1, Brain Ig-Like Molecule with Tyrosine-Based Activation Motifs, Bit, CD172 Antigen-Like Family Member A, Inhibitory Feceptor SHPS-1, Macrophage Fusion Receptor, MyD-1 Antigen, Signal-Regulatory Protein Alpha-1, Sirp-Alpha-1, Signal-Regulatory Protein Alpha-2, Sirp-Alpha-2, Signal-Regulatory Protein Alpha-3, Sirp-Alpha-3, p84, CD172a, SIRPA, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP

Poids moléculaire: 39.9 kDa

UniProt: P78324