DLL4 Protein (AA 27-524) (Fc Tag)

N° du produit ABIN7274485

Détail du produit

Antigène: DLL4 (delta-Like 4 (DLL4))

Type de proteíne: Recombinant

Attributs du protein: AA 27-524

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette DLL4 protéine est marqué à la Fc Tag.

Fonction: Human DLL4 Protein

Séquence: Ser27-Pro524

Attributs du produit: Recombinant Human DLL4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser27-Pro524.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DLL4, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA. The affinity constant of 0.48 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: DLL4 (delta-Like 4 (DLL4))

Autre désignation: DLL4 (DLL4 Produits)

Synonymes: hdelta2 Protein, Delta4 Protein, si:ch211-244p18.5 Protein, zgc:136596 Protein, delta like canonical Notch ligand 4 Protein, delta-like 4 (Drosophila) Protein, DLL4 Protein, Dll4 Protein, dll4 Protein

Sujet: Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.

Poids moléculaire: 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Tris-Bis PAGE result.

UniProt: Q9NR61

Pathways: Signalisation Notch