GDNF Protein (AA 78-211)
-
- Antigène Voir toutes GDNF Protéines
- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 78-211
-
Origine
- Humain
-
Source
- Escherichia coli (E. coli)
- Fonction
- Human GDNF Protein
- Séquence
- Ser78-Ile211
- Attributs du produit
- Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human GDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
-
- Antigène
- GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
- Autre désignation
- GDNF (GDNF Produits)
- Synonymes
- gdnf Protein, GDNF Protein, AI385739 Protein, gdnfa Protein, hfb1-gdnf Protein, ATF1 Protein, ATF2 Protein, HFB1-GDNF Protein, HSCR3 Protein, glial cell derived neurotrophic factor Protein, glial cell derived neurotrophic factor a Protein, glial cell line derived neurotrophic factor Protein, glial cell derived neurotrophic factor L homeolog Protein, Gdnf Protein, gdnfa Protein, GDNF Protein, gdnf.L Protein
- Sujet
- Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.
- Poids moléculaire
- 15.06 kDa same as Tris-Bis PAGE result.
- Pathways
- Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
-