Cette Galectin 3 protéine est marqué à la His tag.
Fonction
Human Galectin 3 Protein
Séquence
Ala2-Ile250
Attributs du produit
Recombinant Human Galectin 3 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Ala2-Ile250.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Galectin 3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA. See testing image for detail.
LGALS3
Origine: Cynomolgus
Hôte: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Agent conservateur
Other preservative
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).
Poids moléculaire
27.1 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.