GPC1 Protein (AA 24-530) (His tag)

N° du produit ABIN7274773

Détail du produit

Antigène: GPC1 (Glypican 1 (GPC1))

Type de proteíne: Recombinant

Attributs du protein: AA 24-530

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette GPC1 protéine est marqué à la His tag.

Fonction: Human Glypican 1/GPC1 Protein

Séquence: Asp24-Ser530

Attributs du produit: Recombinant Human Glypican 1/GPC1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp24-Ser530.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FGF basic at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 21.9ng/ml determined by ELISA. See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: GPC1 (Glypican 1 (GPC1))

Autre désignation: Glypican 1 (GPC1 Produits)

Synonymes: gpc1 Protein, zgc:63947 Protein, GPC1 Protein, Glypican-1 Protein, glypican Protein, AI462976 Protein, HSPG M12 Protein, HSPGM12 Protein, glypican 1b Protein, glypican 1 Protein, gpc1b Protein, GPC1 Protein, gpc1 Protein, Gpc1 Protein

Sujet: CAR-T cells targeting glypican-1 (GPC1)-specific human and murine CAR-T cells generated from our original anti-human/mouse GPC1 antibody showed strong antitumor effects in xenogeneic and syngeneic mouse models, respectively. Importantly, the murine CAR-T cells enhanced endogenous T cell responses against a non-GPC1 tumor antigen through the mechanism of antigen-spreading and showed synergistic antitumor effects with anti-PD-1 antibody without any adverse effects in syngeneic models.

Poids moléculaire: 57.1 kDa. Due to glycosylation, the protein migrates to 70-135 kDa based on Tris-Bis PAGE result.

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation