IL-5 Protein (AA 20-134) (His-Avi Tag,Biotin)

N° du produit ABIN7274955

Détail du produit

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Type de proteíne: Recombinant

Attributs du protein: AA 20-134

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette IL-5 protéine est marqué à la His-Avi Tag,Biotin.

Fonction: Biotinylated Human IL-5 Protein

Séquence: Ile20-Ser134

Attributs du produit: Recombinant Biotinylated Human IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ile20-Ser134.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human IL-5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-5 R alpha, hFc Tag with the EC50 of 39ng/ml determined by ELISA. The affinity constant of 7.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: IL-5 (IL5 Produits)

Synonymes: EDF Protein, IL-5 Protein, TRF Protein, Il-5 Protein, IL5 Protein, interleukin 5 Protein, IL5 Protein, Il5 Protein

Sujet: IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).

Poids moléculaire: 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.

UniProt: P05113

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour