Klotho beta Protein (KLB) (His tag)
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- Antigène Voir toutes Klotho beta (KLB) Protéines
- Klotho beta (KLB)
- Type de proteíne
- Recombinant
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette Klotho beta protéine est marqué à la His tag.
- Séquence
- Met30-Thr983
- Pureté
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Beta Klotho at 2μg/ml (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 38.5ng/ml determined by ELISA. The affinity constant of 0.54nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Klotho beta (KLB) (AA 53-994) protein (His tag)
KLB Origine: Souris Hôte: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Klotho beta (KLB) (AA 1-1044) protein (Strep Tag)Crystallography grade KLB Origine: Humain Hôte: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Klotho beta (KLB) (AA 1-1043) protein (Strep Tag)Crystallography grade KLB Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Klotho beta (KLB) (AA 1-996) protein (His tag)Crystallography grade KLB Origine: Humain Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Klotho beta (KLB) (AA 1-994) protein (His tag)Crystallography grade KLB Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- 4 °C,-80 °C
- Stockage commentaire
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- Klotho beta (KLB)
- Autre désignation
- Beta Klotho (KLB Produits)
- Synonymes
- KLB Protein, BKL Protein, AV071179 Protein, betaKlotho Protein, Klb-ps1 Protein, RGD1308227 Protein, klotho beta Protein, KLB Protein, Klb Protein
- Sujet
- BetaKlotho, beta-klotho, BKL, KLB, klotho beta like, Klotho beta, MGC142213,Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
- Poids moléculaire
- 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
- UniProt
- Q86Z14
- Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Growth Factor Binding
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