KREMEN2
(Kringle Containing Transmembrane Protein 2 (KREMEN2))
Type de proteíne
Recombinant
Attributs du protein
AA 26-364
Origine
Cynomolgus
Source
HEK-293 Cells
Purification/Conjugué
Cette KREMEN2 protéine est marqué à la His tag.
Fonction
Cynomolgus Kremen-2 Protein
Séquence
Gly26-Ala364
Attributs du produit
Recombinant Cynomolgus Kremen-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly26-Ala364.
Pureté
> 95 % as determined by Tris-Bis PAGE
Stérilité
0.22 μm filtered
niveau d'endotoxine
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus Kremen-2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Kremen-2 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
KREMEN2
Origine: Humain
Hôte: HEK-293 Cells
Recombinant
>95 % as determined by reduced SDS-PAGE.
Active
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
Stock
-20 °C,-80 °C
Stockage commentaire
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Date de péremption
12 months
Antigène
KREMEN2
(Kringle Containing Transmembrane Protein 2 (KREMEN2))
Kremen2 (Krm2) plays an important role in embryonic development, bone formation, and tumorigenesis as a crucial regulator of classical Wnt/β-catenin signaling pathway. Compared to para-cancerous tissues, Krm2 was significantly up-regulated in gastric cancer tissues and was positively correlated with the pathological grade of gastric cancer patients. Krm2 can be a potent candidate for designing of targeted therapy.
Poids moléculaire
37.04 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.