LRRC15 Protein (AA 22-538) (His-Avi Tag,Biotin)
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- Antigène Tous les produits LRRC15
- LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 22-538
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Origine
- Humain
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Source
- CHO Cells
- Purification/Conjugué
- Cette LRRC15 protéine est marqué à la His-Avi Tag,Biotin.
- Fonction
- Biotinylated Human LRRC15/LIB Protein
- Séquence
- Tyr22-Gly538
- Attributs du produit
- Recombinant Biotinylated Human LRRC15/LIB Protein is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Tyr22-Gly538.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-LRRC15 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of 38.3ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- LRRC15 (Leucine Rich Repeat Containing 15 (LRRC15))
- Autre désignation
- LRRC15 (LRRC15 Produits)
- Synonymes
- cb894 Protein, zgc:158286 Protein, LIB Protein, 5430427N11Rik Protein, Lib Protein, leucine rich repeat containing 15 Protein, leucine-rich repeat-containing protein 15 Protein, carboxypeptidase N subunit 2 Protein, LRRC15 Protein, lrrc15 Protein, CpipJ_CPIJ004946 Protein, CpipJ_CPIJ005191 Protein, CpipJ_CPIJ007745 Protein, CPN2 Protein, Lrrc15 Protein
- Sujet
- The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation. LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta, small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
- Poids moléculaire
- 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
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