MCAM Protein (AA 24-559) (His tag)
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- Antigène Voir toutes MCAM Protéines
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 24-559
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Origine
- Humain
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Source
- HEK-293 Cells
- Purification/Conjugué
- Cette MCAM protéine est marqué à la His tag.
- Fonction
- Human MUC18/CD146 Protein
- Séquence
- Val24-Gly559
- Attributs du produit
- Recombinant Human MUC18/CD146 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Gly559.
- Pureté
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human MUC18, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Stock
- -20 °C,-80 °C
- Stockage commentaire
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Date de péremption
- 12 months
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- Antigène
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- Autre désignation
- MUC18 (MCAM Produits)
- Synonymes
- CD146 Protein, MUC18 Protein, Muc18 Protein, 1-gicerin Protein, AV025631 Protein, CD149 Protein, s-endo Protein, s-gicerin Protein, cd146 Protein, gicerin Protein, id:ibd3512 Protein, mcam Protein, MCAM Protein, melanoma cell adhesion molecule Protein, melanoma cell adhesion molecule b Protein, MCAM Protein, Mcam Protein, mcamb Protein
- Sujet
- METCAM/MUC18 is an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family. It can carry out common functions of CAMs which is to perform intercellular interactions and interaction of cell with extracellular matrix in tumor microenvironment, to interact with various signaling pathways and to regulate general behaviors of cells.The above positive correlation is fortuitous, actually METCAM/MUC18 acts as a tumor and metastasis suppressor for the ovarian carcinoma cells.
- Poids moléculaire
- 60.8 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
- UniProt
- P43121
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