NCR3 Protein (AA 19-138) (Fc Tag)

N° du produit ABIN7275350

Détail du produit

Antigène: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Type de proteíne: Recombinant

Attributs du protein: AA 19-138

Origine: Humain

Source: HEK-293 Cells

Purification/Conjugué: Cette NCR3 protéine est marqué à la Fc Tag.

Fonction: Human NKp30/NCR3/CD337 Protein

Séquence: Leu19-Thr138

Attributs du produit: Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu19-Thr138.

Pureté: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human NKp30, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. The affinity constant of 23.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Stock: -20 °C,-80 °C

Stockage commentaire: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Autre désignation: NKp30 (NCR3 Produits)

Synonymes: MGC133750 Protein, 1C7 Protein, CD337 Protein, LY117 Protein, MALS Protein, NKp30 Protein, Nkp30 Protein, natural cytotoxicity triggering receptor 3 Protein, NCR3 Protein, Ncr3 Protein

Sujet: NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.

Poids moléculaire: 39.8 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process